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Mapping Immunity Across Time, Space and Disease State | Tom Ashhurst, PhD

Learn how Thomas Ashhurst, PhD, of the Sydney Cytometry Facility applied mass cytometry to immune cell type characterization. West Nile virus and Zika virus are responsible for central nervous system diseases including encephalitis, Guillain-Barré syndrome and congenital neurological abnormalities in the developing fetus. Hear about the use of highly multiplexed, quantitative and spatially resolved tumor tissue analyses using Imaging Mass Cytometry™ (IMC™) and high-dimensional flow cytometry to analyze the cellular composition of virally infected mouse tissue.


In this webinar presented in late spring 2020, Ashhurst presents an update to a session presented at the 2019 IMC User Group Meeting and discusses:

- The current state of single-cell cytometry and imaging technologies
- How these technologies and computational analysis are applied to map the dynamic immune response to viral inflammation
- Using the Hyperion™ Imaging System in dual mode capacity for the characterization of the evolving immune response over time
• Using high-parameter mass cytometry for broad profiling of the murine hemopoietic system during inflammation
• Performing IMC to advance the spatial understanding of tissue microenvironment at the single-cell level


Tom Ashhurst, PhD
High‐Dimensional Cytometry Specialist
Sydney Cytometry Facility

Thomas Ashhurst is a High‐Dimension Cytometry Specialist at the Sydney Cytometry Facility. The core research facility for cytometry, cell sorting and imaging is associated with the University of Sydney and is committed to the development and transfer of cytometry expertise and techniques, solidifying the role of cytometry as a major modality in biological and biomedical research. Ashhurst is also a co-editor of the 2019 Methods in Molecular Biology book, Mass Cytometry: Methods and Protocols.