Comprehensively characterize tissue microenvironment in spatial context with Imaging Mass Cytometry™

July 2020

Imaging Mass Cytometry™ Using CyTOF®
Eric Swanson, PhD, Field Applications Scientist, CyTOF, Fluidigm

Biomarker discovery efforts in translational medicine are increasingly reliant on antibody-mediated approaches for protein detection in both suspension and fixed tissue sections.

The Hyperion™ Imaging System enables simultaneous interrogation of 4 to 37 protein markers using proven CyTOF® technology together with imaging capability, facilitating Imaging Mass Cytometry™ (IMC™) applications. The use of highly pure metal labels on antibodies in place of fluorescent tags provides a solution to the current challenges in multiplexed tissue imaging by separating signals based on differences in mass instead of wavelength to overcome the limitations of fluorescence-based detection modalities.

The Hyperion Imaging System makes it possible to deeply interrogate tissues and tumors while preserving the information in tissue architecture and cellular morphology, ideal for characterization of the tissue microenvironment across a breadth of disease research areas. This presentation will provide an overview of the Hyperion Imaging System and present data to demonstrate how an Imaging Mass Cytometry approach can be used to uncover new biomarkers and cellular interactions on fixed tissue sections.