Deep Immunophenotyping of Cancer Microenvironments by Imaging Mass Cytometry™ | Noel de Miranda, PhD

Multiplex immunophenotyping technologies are indispensable for a deeper understanding of biological systems. Until recently, high-dimensional cellular analyses implied the loss of tissue context because most were performed in single-cell suspensions. In this webinar Noel de Miranda introduces how the advent of Imaging Mass Cytometry™ (IMC™) has enabled highly multiplexed immunohistochemistry of frozen and formalin-fixed, paraffin-embedded (FFPE) tissues using proven CyTOF® technology.


In this presentation from the July 2020 Fluidigm Oncology and Infectious Disease Virtual Summit, de Miranda discusses:

- Details of how his team developed an optimized workflow for maximum antibody performance
- Insights as to how IMC can be implemented in other laboratories
- Methodological challenges that were overcome to develop a large antibody panel to preserve signal intensity and specificity of antigen detection

Hear de Miranda outline why the IMC panel developed here is an excellent immune monitoring tool that can be readily applied in the context of research and clinical trials.


Noel de Miranda, PhD
Principal Investigator
Leiden University Medical Center

Noel de Miranda is a Principal Investigator in the Department of Pathology at the Leiden University Medical Center and is the Project Leader for the Immunogenomics group. His group aims to develop innovative therapies focused on stimulating the immune system to recognize and eliminate cancer cells. Currently the research focuses on the development of neoantigen-targeted therapies for patients diagnosed with cancers with low-mutational burden and the deep immuno-phenotyping of colorectal and pancreatic cancers and discovery of immune cell subsets with anti-tumor activity.